Think you’ve got your head wrapped around DNA Structure, Replication, and Technology? Put your knowledge to
the test. Good luck — the Stickman is counting on you!
Q. We never talked about other enzymes that modify histones affecting packaging of genes. This leads to inhibition of gene expression or activation of gene expression, depending on how open DNA packaging is, where more packaging = gene repression. Can you fill in the table below knowing the information that you know? The answer is "yes". (answers in bold) Which of the following enzymes is MOST likely to be responsible for adding acetyl groups to histones?
Histone methyltransferase
Ubiquitin ligase
Histone kinase
Histone deacetylase
Histone acetyltransferase
Q. Which of the following enzymes is most likely to increase gene expression?
An enzyme that adds a methyl group to histones
An enzyme that adds a negative phosphate group to histones
An enzyme that removes an acetyl group from histones
An enzyme that adds a methyl group to DNA
An enzyme that removes a phosphate group from histones
Q. What is the likely function of arginine deiminase?
Adds a methyl group to DNA
Adds a phosphate group to DNA
Removes an imino group from arginine
Adds an arginine to DNA
Removes a methyl group from DNA
Q. 
Given the picture (A) above, in which stage of the cell cycle is the cell shown?
Prophase
Anaphase
Telophase/Cytokinesis
Interphase
Metaphase
Q.
Given the picture (B) above, in which stage of the cell cycle is the cell shown?
Prophase
Anaphase
Telophase/Cytokinesis
Interphase
Metaphase
Q.
Given the picture (C) above, in which stage of the cell cycle is the cell shown?
Prophase
Anaphase
Telophase/Cytokinesis
Interphase
Metaphase
Q.
Given the picture (D) above, in which stage of the cell cycle is the cell shown?
Prophase
Anaphase
Telophase/Cytokinesis
Interphase
Metaphase
Q. Which of the following would be the best way to insert a PCR product into a vector?
Digest vector with enzymes that make blunt ends and ligate in the PCR product.
PCR amplify the gene from the flanking DNA sequence that includes unique restriction enzyme sites, and then digest it with a restriction enzyme and ligate.
Put a restriction enzyme cleavage site at the 5´ end of both primers, PCR amplify gene product, and then digest it with a restriction enzyme and ligate.
All of the above would be good strategies.
All of the above are terrible ideas (None of the above).
Q. Which of the following is only found in RNA?
Double-stranded base pairing
Lagging strand
Methylated cytosine
5´-CTTGGATCCGGTACGT-3´
5´-CUUGGAUCCGGUACGU-3´
Q. Which of the following would be the best way to genetically modify a corn plant that carries a gene that is >20 kb in length?
Put the gene in a cosmid with Ti sequences, and then transform with Agrobacterium.
Put the gene in a cosmid with cos sequences, and then transform with Agrobacterium.
Put the gene in a plasmid with Ti sequences, and then infect with a retrovirus.
Put the gene in a cosmid with cos sequences, and then infect with a retrovirus.
Put the gene in a BAC with Ti sequences, and then microinject into embryo.