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Molecular Genetics Videos 21 videos
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Molecular Genetics: Electrophoresis 201 Views
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Description:
In this video from our course on molecular genetics, learn all about electrophoresis.
Transcript
- 00:00
Thank you We sneak in Molecular genetics Electro for e
- 00:10
says a lot Okay we're on dna technology with dr
- 00:16
ruth tenant so the dna then has to be sort
- 00:20
of separated or segmented and managed in various ways Talk
- 00:24
us through the process and you know bass in the
Full Transcript
- 00:26
process of electrophoresis How does all that kind of come
- 00:29
together Yeah so let her freeze is really useful tool
- 00:33
It is basically a way of separating dna by size
- 00:35
using an electric field so you load you add your
- 00:39
dna into an aggro gel which kind of feels like
- 00:41
god If you zoom in really close on it looks
- 00:45
like a spider web so it's all like tons of
- 00:47
running all over what you do is you at an
- 00:49
electric field that is negative where you put the dna
- 00:51
and positive at the other end and the dna which
- 00:54
is negatively charged What kind of run down gel toward
- 00:56
the positive is exactly like that Yep And a smaller
- 01:00
piece of dna the faster it's gonna be able to
- 01:02
make it through that web cool it separate things And
- 01:07
we've done this after we've chopped it up with restriction
- 01:09
enzymes no and then we study the patterns a little
- 01:14
bit like you you know collide adams You'll look at
- 01:17
how they smash together and i'll give you characteristics of
- 01:20
the medium in san And someone is that it was
- 01:22
sort of looking at the the trail of a corpse
- 01:25
of you know sort of what what's happened in this
- 01:27
collision Yes So there's a bunch of different uses So
- 01:29
once you got those pieces separated one thing you do
- 01:31
is actually cut them out of the jail and used
- 01:33
them for other purposes But you could use them for
- 01:35
forensic So you could have a pattern of dna from
- 01:37
a suspect and a pattern of dna from the scene
- 01:39
And you could compare what they look like what the
- 01:41
fingerprints look like Wow Cool So we cut him out
- 01:44
of the spider web gel and we want to use
- 01:47
him for something else Is this you know i think
- 01:49
i remember reading google article about our being able to
- 01:52
we try to grow frog and grow the whole frog
- 01:55
We grew an arm like is it where you can
- 01:58
cut them out and grow cool stuff And or you
- 02:02
know cases It's not super dramatic So you might have
- 02:05
a gene that controls some small thing that you could
- 02:07
put into a new organism And it could give it
- 02:09
a new traits For example like you could have a
- 02:10
plant that normally is you know not resistant to us
- 02:13
or insect And then you look into it And now 00:02:16.513 --> [endTime] it is What's the process of electro for recess
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