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Molecular Genetics: Electrophoresis 201 Views


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Description:

In this video from our course on molecular genetics, learn all about electrophoresis.


Transcript

00:00

Thank you We sneak in Molecular genetics Electro for e

00:10

says a lot Okay we're on dna technology with dr

00:16

ruth tenant so the dna then has to be sort

00:20

of separated or segmented and managed in various ways Talk

00:24

us through the process and you know bass in the

00:26

process of electrophoresis How does all that kind of come

00:29

together Yeah so let her freeze is really useful tool

00:33

It is basically a way of separating dna by size

00:35

using an electric field so you load you add your

00:39

dna into an aggro gel which kind of feels like

00:41

god If you zoom in really close on it looks

00:45

like a spider web so it's all like tons of

00:47

running all over what you do is you at an

00:49

electric field that is negative where you put the dna

00:51

and positive at the other end and the dna which

00:54

is negatively charged What kind of run down gel toward

00:56

the positive is exactly like that Yep And a smaller

01:00

piece of dna the faster it's gonna be able to

01:02

make it through that web cool it separate things And

01:07

we've done this after we've chopped it up with restriction

01:09

enzymes no and then we study the patterns a little

01:14

bit like you you know collide adams You'll look at

01:17

how they smash together and i'll give you characteristics of

01:20

the medium in san And someone is that it was

01:22

sort of looking at the the trail of a corpse

01:25

of you know sort of what what's happened in this

01:27

collision Yes So there's a bunch of different uses So

01:29

once you got those pieces separated one thing you do

01:31

is actually cut them out of the jail and used

01:33

them for other purposes But you could use them for

01:35

forensic So you could have a pattern of dna from

01:37

a suspect and a pattern of dna from the scene

01:39

And you could compare what they look like what the

01:41

fingerprints look like Wow Cool So we cut him out

01:44

of the spider web gel and we want to use

01:47

him for something else Is this you know i think

01:49

i remember reading google article about our being able to

01:52

we try to grow frog and grow the whole frog

01:55

We grew an arm like is it where you can

01:58

cut them out and grow cool stuff And or you

02:02

know cases It's not super dramatic So you might have

02:05

a gene that controls some small thing that you could

02:07

put into a new organism And it could give it

02:09

a new traits For example like you could have a

02:10

plant that normally is you know not resistant to us

02:13

or insect And then you look into it And now 00:02:16.513 --> [endTime] it is What's the process of electro for recess

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